1P4O
Structure of Apo unactivated IGF-1R KInase domain at 1.5A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.950, 85.560, 78.880 |
Unit cell angles | 90.00, 99.10, 90.00 |
Refinement procedure
Resolution | 6.000 * - 1.500 |
Rwork | 0.209 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 * |
RMSD bond angle | 1.220 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.079 | 0.368 * |
Total number of observations | 1497973 * | |
Number of reflections | 98666 | 5844 * |
<I/σ(I)> | 19 | 1.7 |
Completeness [%] | 90.0 | 53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 8% PEG 8k, 0.1M LiCl, 10% ethylene glycol, 10% glycerol and 0.1M Tris-HCl buffer, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | dithiothreitol | 5 (mM) | |
4 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
5 | 1 | reservoir | PEG8000 | 8 (%) | |
6 | 1 | reservoir | 0.1 (M) | ||
7 | 1 | reservoir | ethylene glycol | 10 (%) | |
8 | 1 | reservoir | glycerol | 10 (%) |