1P4E
Flpe W330F mutant-DNA Holliday Junction Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-03-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.760, 116.715, 142.053 |
| Unit cell angles | 90.00, 97.64, 90.00 |
Refinement procedure
| Resolution | 22.000 * - 2.700 |
| Rwork | 0.236 |
| R-free | 0.27500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | Flpe-DNA complex pdb. 1M6X |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.210 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.043 | |
| Number of reflections | 68765 | |
| Completeness [%] | 96.1 | 90.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 277 | PEG 5KMME, Hepes, xylitol, sodium chloride, calcium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 5000 | ||
| 2 | 1 | 1 | H2O | ||
| 3 | 1 | 1 | xylitol | ||
| 4 | 1 | 1 | sodium chlorid | ||
| 5 | 1 | 1 | calcium chloride | ||
| 6 | 1 | 2 | PEG 5000 | ||
| 7 | 1 | 2 | calcium chloride | ||
| 8 | 1 | 2 | H2O |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA substrate | 4 (mM) | |
| 10 | 1 | reservoir | xylitol | 20 (%) | |
| 11 | 1 | reservoir | 100 (mM) | ||
| 12 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 13 | 1 | reservoir | 10 (mM) | ||
| 2 | 1 | drop | protein | 120 (nm) | |
| 3 | 1 | drop | taps | 25 (mM) | pH8.0 |
| 4 | 1 | drop | 200 (mM) | ||
| 5 | 1 | drop | glycerol | 12 (%) | |
| 6 | 1 | drop | EDTA | 2 (mM) | |
| 7 | 1 | drop | bovine serum albumin | 1 (mg/ml) | |
| 8 | 1 | reservoir | HEPES | 20 (mM) | pH7.0 |
| 9 | 1 | reservoir | PEG5000 MME | 11 (%) |
