1P4D
F factor TraI Relaxase Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2003-02-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9713 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 128.219, 128.219, 121.184 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.600 |
| R-factor | 0.238 |
| Rwork | 0.233 |
| R-free | 0.27800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.760 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.054 * | 0.337 * |
| Number of reflections | 34650 | |
| <I/σ(I)> | 43.3 | |
| Completeness [%] | 96.8 * | 92.3 |
| Redundancy | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.25 * | 294 | Larkin, C., (2003) Acta Crystallogr., D59, 1514. * |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.25 * | 294 | Larkin, C., (2003) Acta Crystallogr., D59, 1514. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | Tris | 25 (mM) | pH8.25 |
| 3 | 1 | drop | EDTA | 0.1 (mM) | |
| 4 | 1 | drop | beta-mercaptoethanol | 0.5 (mM) | |
| 5 | 1 | drop | 50 (mM) |
