1P3R

Crystal structure of the phosphotyrosin binding domain(PTB) of mouse Disabled 1(Dab1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-08
Detector	MARRESEARCH
Wavelength(s)	1.02466
Spacegroup name	H 3 2
Unit cell lengths	128.150, 128.150, 272.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.100
Rwork	0.223
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m7e
RMSD bond length	0.008
RMSD bond angle	1.310 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.100	2.300 *
Rmerge	0.084 *
Total number of observations	2717217 *
Number of reflections	49672
<I/σ(I)>	54.7
Completeness [%]	97.1	99.4 *
Redundancy	54.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	18 *	sodium formate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	20 (mM)	pH7.5
2	1	drop	dithiothreitol	1 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop	protein	20 (mg/ml)
5	1	reservoir	sodium formate	3.5 (M)
6	1	reservoir	HEPES	50 (mM)	pH8.5