1P2C

crystal structure analysis of an anti-lysozyme antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-02-10
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	44.660, 73.750, 83.780
Unit cell angles	66.59, 74.74, 85.44

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.204
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D44.1 Fab
RMSD bond length	0.006 *
RMSD bond angle	1.395 *
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	2.000	2.070
High resolution limit [Å]	2.000 *	2.000
Rmerge	0.013	0.036
Number of reflections	60488
Completeness [%]	95.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	298	PEG 1000, CaCl2, pH 4.6, vapor diffusion, temperature 298.K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris	5 (mM)	pH7.5
3	1	reservoir	PEG1000	28 (%)
4	1	reservoir		0.2 (M)	pH4.6