1P28
The crystal structure of a pheromone binding protein from the cockroach Leucophaea maderae in complex with a component of the pheromonal blend: 3-hydroxy-butan-2-one.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-04 |
Detector | MARRESEARCH |
Wavelength(s) | 0.919214 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.217, 62.210, 45.117 |
Unit cell angles | 90.00, 92.47, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.14508 |
Rwork | 0.141 |
R-free | 0.14100 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.500 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.046 * | 0.182 * |
Number of reflections | 23078 | |
<I/σ(I)> | 10.1 | 3.7 |
Completeness [%] | 97.0 | 93.1 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 5.6 | 293 | Sulzenbacher, G., (2002) Acta Cryst., D58, 2109. * |