1P1Q

Crystal structure of the GluR2 ligand binding core (S1S2J) L650T mutant in complex with AMPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	110
Detector technology	CCD
Collection date	2001-03-19
Detector	ADSC QUANTUM 4
Wavelength(s)	0.920
Spacegroup name	P 21 21 2
Unit cell lengths	113.942, 163.642, 47.116
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.222
Rwork	0.217
R-free	0.27000
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006 *
RMSD bond angle	1.310 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.039	0.070
Number of reflections	55769
<I/σ(I)>	25.3
Completeness [%]	91.9	64.9
Redundancy	3.98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	4 *	PEG8000, cacodylate, zinc acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	12-16 (%)
2	1	reservoir	zinc acetate	0.1-0.3 (M)
3	1	reservoir	cacodylate	0.1 (M)	pH6.5