1P1G
MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) WITH PRO-1 MUTATED TO GLY-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 133 |
Collection date | 1997-12 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 95.810, 95.810, 104.160 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
Rwork | 0.227 |
R-free | 0.27000 |
Structure solution method | OTHER |
RMSD bond length | 0.009 |
RMSD bond angle | 1.550 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.790 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.049 * | 0.105 * |
Total number of observations | 50378 * | |
Number of reflections | 18195 * | |
<I/σ(I)> | 6.5 | |
Completeness [%] | 93.7 | 94.8 |
Redundancy | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-15 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
3 | 1 | reservoir | HEPES | 0.1 (M) | |
4 | 1 | reservoir | PEG400 | 2 (%) |