1P0B
Crystal Structure Of tRNA-Guanine Transglycosylase (TGT) From Zymomonas mobilis Complexed With Archaeosine Precursor, Preq0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-03-06 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.620, 65.540, 70.330 |
Unit cell angles | 90.00, 96.46, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.20000 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1pud |
RMSD bond length | 0.008 |
RMSD bond angle | 22.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.104 * | 0.562 * |
Total number of observations | 163734 * | |
Number of reflections | 43081 | |
Completeness [%] | 95.6 | 92.2 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 25 * | Romier, C., (1996) Proteins: Struct.,Funct., Genet., 24, 516. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris-HCl | 100 (mM) | |
2 | 1 | reservoir | PEG8000 | 13 (%) | |
3 | 1 | reservoir | dithiothreitol | 1 (mM) | |
4 | 1 | reservoir | agarose | 0.2 (%) | |
5 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
6 | 1 | drop | 2 (M) | ||
7 | 1 | drop | dithiothreitol | 1 (mM) | |
8 | 1 | drop | protein | 12 (mg/ml) |