1OZB
Crystal Structure of SecB complexed with SecA C-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 114.500, 114.500, 286.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.800 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.26800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SecB coordinate |
| RMSD bond length | 0.008 |
| RMSD bond angle | 25.200 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.056 * | 0.294 * |
| Total number of observations | 323553 * | |
| Number of reflections | 47436 | |
| <I/σ(I)> | 31.3 | 6 |
| Completeness [%] | 99.2 | 98.1 |
| Redundancy | 6.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.7 | 277 | PEG 2000, magnesium sulfate, benzamidine hydrochloride, glycine , pH 9.7, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17.4 (mg/ml) | |
| 2 | 1 | reservoir | PEG2000 | 24.5 (%(w/v)) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | glycine | 100 (mM) | pH9.7 |
