1OYU
Long-Distance conformational changes in a protein engineered by modulated sequence duplication
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 170 |
Detector technology | IMAGE PLATE |
Collection date | 2001-11-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.99 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 60.350, 60.350, 213.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.000 - 2.500 |
R-factor | 0.219 |
Rwork | 0.209 |
R-free | 0.32400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lzm |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.620 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.072 * | |
Number of reflections | 12441 * | |
<I/σ(I)> | 8.2 | 3.3 |
Completeness [%] | 94.8 | 96.6 |
Redundancy | 6.8 | 5.21 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5.5 | 4 * | 25% poly-ethylene glycol 4000, 50mM phosphate buffer, 0.2mM ammonium acetate, 20% isopropanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 25 (%(w/v)) | |
2 | 1 | reservoir | isopropanol | 20 (%(v/v)) | |
3 | 1 | reservoir | phosphate buffer | 50 (mM) | pH5.5 |
4 | 1 | reservoir | ammonium acetate | 0.2 (mM) |