1OYG

Crystal structure of Bacillus subtilis levansucrase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.18
Spacegroup name	P 21 21 21
Unit cell lengths	51.110, 67.014, 123.681
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.500
R-factor	0.1655
Rwork	0.165
R-free	0.17500 *
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.100 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.500	1.500 *
Rmerge	0.091 *	0.095 *
Total number of observations	683968 *
Number of reflections	67836
Completeness [%]	98.4	98.4 *
Redundancy	5.1	3.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	6.3 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	60 (mg/ml)
2	1	2	diammonium phosphate	1 (mM)
3	1	2	sodium acetate	1 (mM)	pH6.3