1OXX
Crystal structure of GlcV, the ABC-ATPase of the glucose ABC transporter from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.282, 48.427, 183.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.500 * - 1.500* |
| R-factor | 0.17449 |
| Rwork | 0.172 |
| R-free | 0.21200 * |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.500 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.075 * | 0.373 * |
| Number of reflections | 62479 | |
| <I/σ(I)> | 14.3 | 2 |
| Completeness [%] | 80.0 | 31.2 |
| Redundancy | 2.93 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 * | Verdon, G., (2002) Acta Crystallogr., D58, 362. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.25 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 15 (%) | |
| 3 | 1 | reservoir | PEG400 | 15 (%) | |
| 4 | 1 | reservoir | Tris | 0.1 (M) | pH8.3 |
| 5 | 1 | reservoir | 0.5 (M) | ||
| 6 | 1 | reservoir | glycerol | 5 (%) |
