1OXJ

Crystal structure of the Smaug RNA binding domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	170
Detector technology	CCD
Collection date	2001-03-01
Detector	SBC-2
Wavelength(s)	0.94207, 0.97941, 0.97927
Spacegroup name	H 3
Unit cell lengths	129.320, 129.320, 33.125
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 1.800
R-factor	0.231
Rwork	0.229
R-free	0.24400
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.010 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	1.800	1.800
Rmerge	0.036	0.330
Total number of observations	151643 *
Number of reflections	19192
<I/σ(I)>	34.9	3.2
Completeness [%]	95.5	74.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	4 *	PEG 4000, Sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8	4 *	PEG 4000, Sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	17 (%)
2	1	reservoir	sodium acetate	220 (mM)
3	1	reservoir	Tris-Cl	100 (mM)	pH8.0