1OXE

Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-11
Detector	MARRESEARCH
Wavelength(s)	1.05
Spacegroup name	P 21 21 21
Unit cell lengths	50.952, 62.768, 69.519
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 1.690 *
R-factor	0.223
Rwork	0.223
R-free	0.24300 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.586 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000 *	1.220
High resolution limit [Å]	1.690 *	1.690 *
Rmerge	0.043 *	0.330 *
Number of reflections	73514
Completeness [%]	90.8 *	83.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	GdFP	9.1 (mg/ml)
3	1	drop	PEG1000	14 (%(w/v))
4	1	reservoir	HEPES	0.1 (M)	pH7.5
5	1	reservoir	PEG8000	5 (%)
6	1	reservoir	ethylene gylcol	10 (%)