1OXB

Complex between YPD1 and SLN1 response regulator domain in space group P2(1)2(1)2(1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	51.730, 74.250, 98.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.300
R-factor	0.219
Rwork	0.214
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1QSP (YPD1)
RMSD bond length	0.012
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.050	0.239 *
Total number of observations	84027 *
Number of reflections	17319 *
<I/σ(I)>	19
Completeness [%]	98.3	94.5
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	296	Choobakc, L., (2003) Acta Cryst., D59, 927. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-12 (mg/ml)
2	1	reservoir	ammonium sulfate	2-3 (M)
3	1	reservoir	sodium acetate	50 (mM)	pH5.3