1OX9
Crystal structure of SspB-ssrA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9500 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.513, 81.524, 131.773 |
| Unit cell angles | 90.00, 92.83, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.245 |
| Rwork | 0.243 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.430 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.990 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.124 * | 0.236 * |
| Total number of observations | 86232 * | |
| Number of reflections | 34398 | |
| Completeness [%] | 93.4 | 89.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 * | 22 * | PEG 8K, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.7 |
| 3 | 1 | drop | 250 (mM) | ||
| 4 | 1 | drop | EDTA | 2 (mM) | |
| 5 | 1 | drop | beta-mercaptoethanol | 2 (mM) | |
| 6 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5 |
| 7 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 8 | 1 | reservoir | PEG8000 | 15-18 (%) |
