1OX8

Crystal structure of SspB

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9785
Spacegroup name	P 43 21 2
Unit cell lengths	60.116, 60.116, 187.984
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.249
Rwork	0.248
R-free	0.28400
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.380
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	DM
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.065 *	0.196 *
Total number of observations	503846 *
Number of reflections	18454
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7 *	22 *	NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)	pH7.7
3	1	drop		250 (mM)
4	1	drop	EDTA	2 (mM)
5	1	drop	beta-mercaptoethanol	2 (mM)
6	1	reservoir	HEPES-KOH	100 (mM)	pH7.5
7	1	reservoir		4.0-4.1 (M)