1OW8

Paxillin LD2 motif bound to the Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-30
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	87.976, 220.780, 97.373
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.500 - 2.850
R-factor	0.23726
Rwork	0.235
R-free	0.27500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDBentry 1K05
RMSD bond length	0.012
RMSD bond angle	2.060 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.500	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.104 *	0.680 *
Total number of observations	82912 *
Number of reflections	22456
<I/σ(I)>	5.1	1.2
Completeness [%]	99.6	99.3
Redundancy	3.7	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	18 *	sodium chloride, glycerol, Hepes, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		3.9 (M)
2	1	reservoir	glycerol	5 (%)
3	1	reservoir	HEPES	100 (mM)	pH6.3