1OW2
STRUCTURE AND MECHANISM OF ACTION OF ISOPENTENYLPYROPHOSPHATE-DIMETHYLALLYLPYROPHOSPHATE ISOMERASE: COMPLEX OF C67A MUTANT WITH EIPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.948, 71.394, 92.381 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 * - 2.000 |
| R-factor | 0.228 * |
| Rwork | 0.228 |
| R-free | 0.28200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hzt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.021 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.230 | 2.090 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.066 | 0.128 |
| Total number of observations | 97958 * | |
| Number of reflections | 32619 * | |
| <I/σ(I)> | 6.2 | 4.7 |
| Completeness [%] | 98.2 | 98.1 * |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 | PEG 2000, MANGANESE CHLORIDE, AMMONIUM SULFATE, pH 5.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG2000 | 16 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | Tris/Maleate | pH5.5 |
