1OVP

LecB (PA-LII) in complex with fructose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-21
Detector	MARRESEARCH
Wavelength(s)	0.9
Spacegroup name	I 2 2 2
Unit cell lengths	53.060, 69.050, 74.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.400
R-factor	0.1833
Rwork	0.182
R-free	0.19700 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.102
RMSD bond angle	1.561
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.061 *	0.087
Total number of observations	155542 *
Number of reflections	26791
Completeness [%]	98.8 *	99.7
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG8000, ammonium sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20 (%)
2	1	reservoir	ammonium sulfate	150 (mM)
3	1	drop	protein	5 (mg/ml)
4	1	drop	Tris	100 (mM)	pH8.0