1OVM

Crystal structure of Indolepyruvate decarboxylase from Enterobacter cloacae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	110
Detector technology	CCD
Collection date	2002-04-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	132.200, 151.620, 107.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.560 - 2.650
R-factor	0.207
Rwork	0.205
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zpd
RMSD bond length	0.007 *
RMSD bond angle	1.388 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.560	2.770
High resolution limit [Å]	2.650	2.650
Rmerge	0.087 *	0.369 *
Total number of observations	315465 *
Number of reflections	63426 *
<I/σ(I)>	7.8	2
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	20 *	PEGmme, Na citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	4 (mg/ml)
2	1	drop	MES/NaOH	20 (mM)	pH6.5
3	1	drop	dithiothreitol	1 (mM)
4	1	reservoir	sodium citrate	0.1 (M)	pH5.0
5	1	reservoir	PEG	8-12 (%(w/v))