1OVM
Crystal structure of Indolepyruvate decarboxylase from Enterobacter cloacae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-04-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 132.200, 151.620, 107.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.560 - 2.650 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zpd |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.388 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.560 | 2.770 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.087 * | 0.369 * |
| Total number of observations | 315465 * | |
| Number of reflections | 63426 * | |
| <I/σ(I)> | 7.8 | 2 |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 20 * | PEGmme, Na citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 4 (mg/ml) | |
| 2 | 1 | drop | MES/NaOH | 20 (mM) | pH6.5 |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.0 |
| 5 | 1 | reservoir | PEG | 8-12 (%(w/v)) |
