1OVL
Crystal Structure of Nurr1 LBD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2001-11-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 80.383, 80.383, 227.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 500.000 - 2.200 |
| R-factor | 0.231 |
| Rwork | 0.217 |
| R-free | 0.25900 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.700 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.072 * | 0.269 |
| Total number of observations | 287222 * | |
| Number of reflections | 82388 | |
| <I/σ(I)> | 6.2 | 2.4 |
| Completeness [%] | 98.7 | 91.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.9 * | 20 * | PEG3350,KBr, Hepes, pH 6.5, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-7 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.9 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | EDTA | 2 (mM) | |
| 5 | 1 | drop | dithiothreitol | 5 (mM) | |
| 6 | 1 | reservoir | PEG3350 | 18 (%(w/v)) | |
| 7 | 1 | reservoir | HEPES | 0.1 (M) | pH6.5 |
| 8 | 1 | reservoir | 0.2 (M) |
