1OVB

THE MECHANISM OF IRON UPTAKE BY TRANSFERRINS: THE STRUCTURE OF AN 18KD NII-DOMAIN FRAGMENT AT 2.3 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 31
Unit cell lengths	41.500, 41.500, 81.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 2.300
R-factor	0.195
Rwork	0.195
RMSD bond length	0.008
RMSD bond angle	0.011 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.044 *
Total number of observations	13822 *
Number of reflections	6535 *
Completeness [%]	90.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	MPD	40 (%(v/v))
3	1	reservoir	Tris-HCl	50 (mM)