1OV9

Crystal structure of the N-terminal dimerisation domain of VicH, the H-NS protein from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9184
Spacegroup name	I 2 2 2
Unit cell lengths	37.085, 49.192, 101.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.258
Rwork	0.233
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ni8
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	EPMR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.085 *	0.570 *
Total number of observations	11950 *
Number of reflections	3894
<I/σ(I)>	7	1.2
Completeness [%]	90.8 *	90.8
Redundancy	3.1	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	18 *	sodium chloride, PEG 3350, potassium iodide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	10 (mM)	pH7.5
2	1	drop		300 (mM)
3	1	drop	protein	20 (mg/ml)
4	1	reservoir		0.2 (M)
5	1	reservoir	PEG3350	20 (%)