1OUV

Helicobacter cysteine rich protein C (HcpC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	110
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	102.982, 46.198, 76.390
Unit cell angles	90.00, 120.95, 90.00

Refinement procedure

Resolution	50.000 - 2.000
Rwork	0.202
R-free	0.24900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1klx
RMSD bond length	0.005
RMSD bond angle	0.971
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.074 *	0.347 *
Number of reflections	21245
<I/σ(I)>	14.37	2.14
Completeness [%]	100.0	100
Redundancy	3.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	30% PEG 4000, 0.2M AMMONIUM ACETATE, 0.1M SODIUM CITRATE, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	30% PEG 4000, 0.2M AMMONIUM ACETATE, 0.1M SODIUM CITRATE, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	reservoir	sodium citrate	0.1 (M)
3	1	reservoir	ammonium acetate	0.2 (M)	pH6.4
4	1	reservoir	PEG4000	30 (%(w/v))