1OUP
Crystal structure of the periplasmic endonuclease Vvn complexed with octamer double stranded DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9065 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.740, 50.110, 89.610 |
| Unit cell angles | 90.00, 97.53, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 2.300 |
| Rwork | 0.191 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SeMet-Vvn |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.350 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.073 * | 0.282 * |
| Total number of observations | 157796 * | |
| Number of reflections | 21165 | |
| <I/σ(I)> | 20.2 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | PEG3350, Calcium acetate, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG3350 | ||
| 2 | 1 | 1 | Calcium acetate | ||
| 3 | 1 | 2 | PEG3350 | ||
| 4 | 1 | 2 | Calcium acetate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 20 (%) | ||
| 2 | 1 | reservoir | calcium acetate | 0.2 (M) |
