1OU5

Crystal structure of human CCA-adding enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-08-21
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	102.520, 102.520, 206.658
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.400
R-factor	0.282
Rwork	0.278
R-free	0.31800
Structure solution method	SIR
RMSD bond length	0.011 *
RMSD bond angle	1.600 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *	3.580
High resolution limit [Å]	3.400	3.400
Rmerge	0.069 *	0.273 *
Total number of observations	46729 *
Number of reflections	17538 *
<I/σ(I)>	9	2.1
Completeness [%]	99.1 *	98.9
Redundancy	2.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5 *	18 *	100mM sodium citrate, 2.2M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)	pH7.5
2	1	drop		400 (mM)
3	1	drop		0.02 (%(w/v))
4	1	drop	dithiothreitol	2 (mM)
5	1	drop	protein	6 (mg/ml)
6	1	reservoir	sodium citrate	100 (mM)	pH5.6
7	1	reservoir	ammonium sulfate	2.2 (M)