1OSZ

MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND AN (L4V) MUTANT OF THE VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1997-12-17
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	136.700, 88.000, 45.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.100
R-factor	0.248
Rwork	0.248
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vaa
RMSD bond length	0.005
RMSD bond angle	1.173
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.076 *	0.290 *
Number of reflections	32332
<I/σ(I)>	15	7.8
Completeness [%]	98.8	98.8
Redundancy	7.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		drop contains equal volume of the reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	phosphate	1.6 (M)
3	1	reservoir	MPD	2.7 (%)
4	1	reservoir	HEPES	0.1 (M)