1OSZ
MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND AN (L4V) MUTANT OF THE VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1997-12-17 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 136.700, 88.000, 45.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.100 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vaa |
RMSD bond length | 0.005 |
RMSD bond angle | 1.173 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.076 * | 0.290 * |
Number of reflections | 32332 | |
<I/σ(I)> | 15 | 7.8 |
Completeness [%] | 98.8 | 98.8 |
Redundancy | 7.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | drop contains equal volume of the reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | phosphate | 1.6 (M) | |
3 | 1 | reservoir | MPD | 2.7 (%) | |
4 | 1 | reservoir | HEPES | 0.1 (M) |