1OSY

Crystal structure of FIP-Fve fungal immunomodulatory protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-07
Detector	PHILLIPS
Wavelength(s)	0.9202
Spacegroup name	P 43 21 2
Unit cell lengths	97.118, 97.118, 61.413
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.020 - 1.700
R-factor	0.18358
Rwork	0.182
R-free	0.21000 *
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.466
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.045	0.187
Total number of observations	464964 *
Number of reflections	32880	3249 *
<I/σ(I)>	28.1	16.7
Completeness [%]	100.0	100
Redundancy	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	21 *	PEG 400, ammonium sulfate, Tris-base, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG400	2.5 (%(w/v))
2	1	reservoir	ammonium sulfate	2.0 (M)
3	1	reservoir	Tris base	0.1 (M)	pH8.5