1OSB
Conjugative Relaxase TrwC in complex with OriT Dna. Metal-free structure.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-20 |
| Detector | MACSCIENCE |
| Spacegroup name | P 65 |
| Unit cell lengths | 147.780, 147.780, 78.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.650 |
| R-factor | 0.1962 |
| Rwork | 0.195 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omh |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.484 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.098 | 0.291 |
| Number of reflections | 28480 | |
| <I/σ(I)> | 7 | 2.5 |
| Completeness [%] | 99.5 | 97.1 |
| Redundancy | 4.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 4 * | 25% PEGMM 2000, 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 296KK |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM ACETATE | ||
| 2 | 1 | 1 | AMMONIUM SULPHATE | ||
| 3 | 1 | 2 | SODIUM ACETATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | reservoir | PEG2000 MME | 25 (%(w/v)) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
