1OS3
Dehydrated T6 human insulin at 100 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-26 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.510, 81.510, 33.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.420 - 1.950 |
| Rwork | 0.216 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ins |
| RMSD bond length | 0.006 |
| RMSD bond angle | 20.200 * |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.420 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.045 | 0.175 |
| Total number of observations | 22524 * | |
| Number of reflections | 6070 | |
| <I/σ(I)> | 15.9 | 3.67 |
| Completeness [%] | 99.6 * | 100 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.3 | 295 | hydrochloric acid, zinc acetate, sodium citrate, acetone, pH 6.3, SLOW COOLING, temperature 295.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | insulin | 5 (mg/ml) | |
| 2 | 1 | 1 | 0.01 (M) | ||
| 3 | 1 | 1 | zinc acetate | 0.007 (M) | |
| 4 | 1 | 1 | sodium citrate | 0.05 (M) | |
| 5 | 1 | 1 | acetone | 17 (%) | pH6.3 |
