1ORQ
X-ray structure of a voltage-dependent potassium channel in complex with an Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.937 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 189.439, 189.439, 150.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.950 - 3.200 |
| R-factor | 0.253 |
| Rwork | 0.253 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb code 1BAF |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.080 * | 0.424 * |
| Number of reflections | 22476 | |
| <I/σ(I)> | 18 | 2 |
| Completeness [%] | 98.5 | 95.2 |
| Redundancy | 5.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 20 * | PEG400, Cadmium chloride, Sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | beta-octylglucoside | 30 (mM) | |
| 2 | 1 | drop | Tris | 20 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | PEG400 | 16-20 (%) | or PEGmme350 |
| 6 | 1 | reservoir | 150-200 (mM) | ||
| 7 | 1 | reservoir | sodium acetate | 100 (mM) |
