1ORO

A FLEXIBLE LOOP AT THE DIMER INTERFACE IS A PART OF THE ACTIVE SITE OF THE ADJACENT MONOMER OF ESCHERICHIA COLI OROTATE PHOSPHORIBOSYLTRANSFERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	54.870, 71.080, 104.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.400
R-factor	0.183
Rwork	0.183
RMSD bond length	0.006
RMSD bond angle	1.460 *
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.500
High resolution limit [Å]	2.400
Rmerge	0.075	0.330 *
Total number of observations	42549 *
Number of reflections	15352	2223 *
Completeness [%]	96.2	96.5 *
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		2.1 (M)
2	1	reservoir	citrate	0.3 (M)