1OR7
Crystal Structure of Escherichia coli sigmaE with the Cytoplasmic Domain of its Anti-sigma RseA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | .9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.049, 56.901, 104.981 |
| Unit cell angles | 90.00, 130.51, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.19879 |
| Rwork | 0.197 |
| R-free | 0.23200 * |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.480 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 * | 0.381 * |
| Total number of observations | 181227 * | |
| Number of reflections | 82833 | |
| <I/σ(I)> | 16.4 | 3.6 |
| Completeness [%] | 98.3 | 92.8 * |
| Redundancy | 2.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 4 * | sodium formate, MES buffer, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | sodium formate | 1.6 (M) | |
| 3 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
