1OQQ
Crystal structure of C73S/C85S mutant of putidaredoxin, a [2Fe-2S] ferredoxin from Pseudomonas putida, at 1.47A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.212, 75.845, 107.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.080 - 1.470 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.21800 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.080 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.049 * | 0.548 |
| Number of reflections | 34883 | |
| <I/σ(I)> | 9.8 | 1.78 |
| Completeness [%] | 98.2 | 98.8 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 298 | ammonium sulfate, sodium citrate, sodium/potassium phosphate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | Bis-Tris propane/acetate | 100 (mM) | pH7.4 |
| 3 | 1 | reservoir | ammonium sulfate | 1.7 (M) | |
| 4 | 1 | reservoir | sodium citrate | 50 (mM) | pH5.9 |
| 5 | 1 | reservoir | sodium potassium phospahte | 300 (mM) |
