1OQN

Crystal structure of the phosphotyrosine binding domain (PTB) of mouse Disabled 1 (Dab1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-20
Detector	MARRESEARCH
Wavelength(s)	1.00465
Spacegroup name	P 21 21 21
Unit cell lengths	59.106, 66.481, 90.528
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
Rwork	0.246
R-free	0.30300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m7e
RMSD bond length	0.009
RMSD bond angle	1.260 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.300
Rmerge	0.077 *
Total number of observations	400319 *
Number of reflections	16118
<I/σ(I)>	27.3
Completeness [%]	99.5
Redundancy	24.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	18 *	PEG 3350, magnesium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	20 (mM)	pH7.5
2	1	drop	dithiothreitol	1 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop	protein	20 (mg/ml)
5	1	reservoir	PEG3350	20 (%(w/v))
6	1	reservoir	tri-lithium citrate	0.2 (M)