1OQM

A 1:1 complex between alpha-lactalbumin and beta1,4-galactosyltransferase in the presence of UDP-N-acetyl-galactosamine

Replaces:  1L7W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-22
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	57.471, 95.677, 100.581
Unit cell angles	90.00, 101.40, 90.00

Refinement procedure

Resolution	19.560 - 2.100
R-factor	0.194
Rwork	0.194
R-free	0.27000 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001 *
RMSD bond angle	0.010 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.060 *
Total number of observations	120498 *
Number of reflections	62285 *
<I/σ(I)>	13.7	2.01
Completeness [%]	90.0 *	76
Redundancy	2.15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6 *	298	Sodium citrate, PEG4000, pH 5.5, LIQUID DIFFUSION, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Gal-T1	20 (mg/ml)
2	1	drop	alpha-lactalbumin	10 (mg/ml)
3	1	reservoir		100 (mM)
4	1	reservoir	sodium citrate	100 (mM)	pH5.6
5	1	reservoir	PEG4000	12.5 (%)