1OQJ

Crystal structure of the SAND domain from glucocorticoid modulatory element binding protein-1 (GMEB1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-13
Detector	MARRESEARCH
Wavelength(s)	0.9185
Spacegroup name	P 21 21 21
Unit cell lengths	40.946, 49.077, 88.903
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 * - 1.550
R-factor	0.194
Rwork	0.194
R-free	0.21000
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	23.000 *
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.450	1.600
High resolution limit [Å]	1.550	1.550
Rmerge	0.047 *	0.108 *
Number of reflections	26235	2085 *
<I/σ(I)>	29.22	7.15
Completeness [%]	98.8	88.6
Redundancy	6.87	2.79

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	100 mM Hepes, 1.4 M sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	0.1 (M)	pH7.5
2	1	reservoir	sodium cirtate	1.4 (M)
3	1	drop	protein	15 (mg/ml)