1OQE
Crystal structure of sTALL-1 with BAFF-R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 233.261, 233.261, 211.286 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.264 |
| Rwork | 0.244 |
| R-free | 0.26500 * |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 27.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 * | 2.450 |
| Rmerge | 0.135 * | 0.600 * |
| Total number of observations | 1415809 * | |
| Number of reflections | 121940 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.3 | 90 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 9 | Liu, Y., (2002) Cell, 108, 383. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | beta-mercaptoethanol | 5 (mM) | |
| 3 | 1 | reservoir | Bicine | 50 (mM) | pH9.0 |
| 4 | 1 | reservoir | 150 (mM) | ||
| 5 | 1 | reservoir | dioxane | 35-38 (%) |
