1OQ4

The Crystal Structure of the Complex between Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean) and Azide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1998-10-28
Detector	MARRESEARCH
Wavelength(s)	0.986
Spacegroup name	P 21 21 21
Unit cell lengths	81.774, 145.210, 192.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.920 - 2.400
R-factor	0.22888
Rwork	0.229
R-free	0.24100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1afr
RMSD bond length	0.010
RMSD bond angle	1.120 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.057 *	0.299 *
Number of reflections	90115
<I/σ(I)>	14.8
Completeness [%]	99.3 *	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4 *	293	70mM Sodium Azide, 12-15% Peg4000, 80mM Cacodylate, 200 mM Magnesium Acetate, 75mM Ammonium Sulphate, 0.2% Octyl glucoside and water exchanged for 20% MPD for freezing, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium azide	70 (mM)
2	1	reservoir	cacodylate	0.08 (M)	pH5.4
3	1	reservoir	magnesium acetate	200 (mM)
4	1	reservoir	ammonium sulfate	75 (mM)
5	1	reservoir	octyl-glucoside	0.2 (%)
6	1	reservoir	PEG4000	12-15 (%)