1OPR

THE CRYSTAL STRUCTURE OF THE OROTATE PHOSPHORIBOSYLTRANSFERASE COMPLEXED WITH OROTATE AND ALPHA-D-5-PHOSPHORIBOSYL-1-PYROPHOSPHATE

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	47.300, 47.300, 216.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.300
R-factor	0.187 *
Rwork	0.187
RMSD bond length	0.017
RMSD bond angle	2.500
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.000
High resolution limit [Å]	2.030 *	2.030 *
Rmerge	0.080 *
Total number of observations	38356 *
Number of reflections	12880	1182 *
Completeness [%]	77.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30-40 (mg/ml)
2	1	drop	orotidine monophosphate	10 (mM)
3	1	drop	ammonium sulfate	65 (%sat)
4	1	drop	NaHepes	70 (mM)
5	1	drop		0.05 (mM)
6	1	drop	beta-mercaptoethanol	10 (mM)
7	1	reservoir	PEG4000	30-40 (%(w/v))
8	1	reservoir	NaHepes	70 (mM)