1OPO
THE STRUCTURE OF CARNATION MOTTLE VIRUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1990-07-15 |
| Detector | HENDRIX-LENTFER |
| Wavelength(s) | 0.96 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 382.600, 382.600, 382.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 3.200 |
| R-factor | 0.183 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tbv |
| RMSD bond length | 0.021 |
| RMSD bond angle | 4.150 |
| Data scaling software | SCALEPACK |
| Phasing software | GAP ((OXFORD)) |
| Refinement software | X-PLOR (2.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 6.000 |
| High resolution limit [Å] | 3.200 |
| Rmerge | 0.082 |
| Number of reflections | 140483 |
| Completeness [%] | 91.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.03 | 298 | 0.1M Tris-maleic (mal)/NaOH, 25% saturated ammonium sulphate, 1.7-heptandiol or PEG 300 were added to lessen the number of pellets, pH 5.03, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 2 | 1 | drop | virus | 40-50 (mg/ml) | |
| 3 | 1 | drop | ammonium sulfate | 10 (%sat) | |
| 4 | 1 | reservoir | Tris-maleic/NaOH | 0.1 (M) | pH5.03 |
| 5 | 1 | reservoir | ammonium sulfate | 25 (%sat) |
