1OPD
HISTIDINE-CONTAINING PROTEIN (HPR), MUTANT WITH SER 46 REPLACED BY ASP (S46D)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-06-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.850, 27.330, 27.600 |
| Unit cell angles | 66.90, 75.71, 70.00 |
Refinement procedure
| Resolution | 8.000 - 1.500 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.23400 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 2.400 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.500 * |
| Rmerge | 0.029 |
| Number of reflections | 10525 |
| Completeness [%] | 93.4 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 * | 14 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | citrate phosphate | 0.1 (M) | |
| 2 | 1 | reservoir | ammonium sulfate | 46-50 (%sat) |
