1OP2
Crystal Structure of AaV-SP-II, a Glycosylated Snake Venom Serine Proteinase from Agkistrodon acutus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.430, 42.830, 44.940 |
| Unit cell angles | 90.00, 99.61, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.100 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 25.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.083 * | 0.208 * |
| Number of reflections | 12259 | |
| Completeness [%] | 92.3 | 79.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 * | 298 | Zhu, Z., (2003) Acta Crystallogr., Sect.D, 59, 547. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | in distilled water |
| 2 | 1 | reservoir | sodium cacodylate | 30 (mM) | pH6.3 |
| 3 | 1 | reservoir | 27 (%(w/v)) |
