1OOK

Crystal Structure of the Complex of Platelet Receptor GPIb-alpha and Human alpha-Thrombin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-02
Detector	ADSC QUANTUM 4
Wavelength(s)	1.033167
Spacegroup name	P 43 21 2
Unit cell lengths	67.649, 67.649, 328.578
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.300
R-factor	0.214
Rwork	0.212
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006 *
RMSD bond angle	1.280 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.066 *	0.292 *
Total number of observations	608108 *
Number of reflections	35360
<I/σ(I)>	38.6	5.2
Completeness [%]	99.7	97.5
Redundancy	17.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	4 *	PEG 6000, Ammonium Phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	2.5 (mM)
2	1	drop		50 (mM)	pH7.4
3	1	drop	protein	25 (mg/ml)
4	1	reservoir	PEG6000	12 (%(w/v))
5	1	reservoir	ammonium phosphate	100 (mM)	pH7.