1ONK
Mistletoe lectin I from viscum album
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.84530 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 107.285, 107.285, 311.773 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.1629 |
| Rwork | 0.160 |
| R-free | 0.22330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mll |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.027 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.052 | 0.396 |
| Number of reflections | 60505 | |
| <I/σ(I)> | 16.05 | |
| Completeness [%] | 96.9 | 93 |
| Redundancy | 6.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 2.5 | 293 | 40% saturated ammonium sulphate, 30% glycerol in 0.1M, pH 2.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
