1ONF
Crystal structure of Plasmodium falciparum Glutathione reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 90.640, 90.640, 123.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.360 - 2.600 |
| R-factor | 0.25981 |
| Rwork | 0.256 |
| R-free | 0.30200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3grs |
| RMSD bond length | 0.019 * |
| RMSD bond angle | 2.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.780 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.094 * | 0.388 * |
| Number of reflections | 16434 | |
| <I/σ(I)> | 13.5 | 4.1 |
| Completeness [%] | 98.7 | 99.9 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | pH 9.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | EDTA | 2 (mM) | pH7.0 |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | reservoir | PEG550 MME | 14 (%(w/v)) | |
| 4 | 1 | reservoir | 70 (mM) | ||
| 5 | 1 | reservoir | Bicine | 70 (mM) | pH8.7-9.0 |
