1OMY
Crystal Structure of a Recombinant alpha-insect Toxin BmKaIT1 from the scorpion Buthus martensii Karsch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2003-01-30 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.241, 36.507, 57.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.720 - 2.110 * |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sn1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.241 |
| Data reduction software | DPS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.720 | 2.110 |
| High resolution limit [Å] | 2.110 * | 2.000 |
| Rmerge | 0.104 * | 0.199 * |
| Total number of observations | 26193 * | |
| Number of reflections | 3971 * | 622 * |
| <I/σ(I)> | 4.7 | 2.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.5 | 6.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.2 * | 296 | Na2HPO4, VAPOR DIFFUSION, HANGING DROP, pH 5.90, temperature 296.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | acetic acid | 10 (mM) | |
| 3 | 1 | drop | 0.02 (%(w/v)) | pH4.2 | |
| 4 | 1 | reservoir | 1.0 (M) | ||
| 5 | 1 | reservoir | 0.02 (%(w/v)) | ||
| 6 | 1 | reservoir | dioxane | 5.0 (%(v/v)) | pH5.9 |
