1OMJ
CRYSTAL STRUCTURE OF A PSYCHROPHILIC ALKALINE PROTEASE FROM PSEUDOMONAS TAC II 18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM02 |
| Synchrotron site | ESRF |
| Beamline | BM02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-09-05 |
| Detector | THOMSON/PRINCETON |
| Wavelength(s) | 1.07 |
| Spacegroup name | H 3 |
| Unit cell lengths | 184.600, 184.600, 37.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.380 |
| R-factor | 0.207 |
| Rwork | 0.195 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kap |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.440 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.600 | 2.510 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.040 * | 0.128 * |
| Total number of observations | 32163 * | |
| Number of reflections | 17980 | |
| <I/σ(I)> | 5.7 | |
| Completeness [%] | 93.2 | 59 |
| Redundancy | 1.8 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 16 * | PEG 4000, ammonium sulphate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 2 | 1 | reservoir | PEG4000 | 22 (%) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
